RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses
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چکیده
منابع مشابه
RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses
A fluent method for gene targeting to establish protein tagged and ligand inducible conditional loss-of-function alleles is described. We couple new recombineering applications for one-step cloning of gRNA oligonucleotides and rapid generation of short-arm (~1 kb) targeting constructs with the power of Cas9-assisted targeting to establish protein tagged alleles in embryonic stem cells at high e...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2016
ISSN: 2045-2322
DOI: 10.1038/srep25529